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KMID : 1023520070300030353
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Korean Journal of Veterinary Service
2007 Volume.30 No. 3 p.353 ~ p.362
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Molecular typing of Listeria monocytogenes using pulsed-field gel electrophoresis (PFGE)
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Chae Hee-Sun
Kim Ju-Young
Kim Yeon-Ha
Yang Yun-Mo
Jin Kyung-Sun
Sin Bang-Woo
Lee Jung-Hark
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Abstract
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A total of 1,354 samples was collected from bovine and porcine carcass from January 2005 to December 2006 in a slaughter house. Twenty five strains(1.8%) of Listeria monocytogenes were isolated from 1,354 samples using selective media. Ten(1.4%) L monocytogenes were isolated from the 677 of bovine carcasses, and 15(2.2%) were isolated from the 677 of porcine carcasses. Among 15 L mono-cytogenes from porcine, 11 siolates were serovars 1/2c, followed by 1/2b (3 strains, 20.0%) and 1/2a(1 strain) Out of 10 bovine samples, positive cases in 1/2a were 9 strains (90.0%), 1/2b were 1 strains(10.0%). PCR primers were selected to amplify a 520-base pair(bp) DNA fragment from the listeolysin O gene (hlyA) of L mono-cytogenes. A 520-bp product was detected in PCR with DNA from L monocytogenes, but not from the other Listeria species tested. A total of 25 L monocytogenes strains were analysed by PFGE after digestion with Apa I. PFGE analysis of genomic DNA showed the fragments ranging in size from 30 to 550 kb, resulting in 14 patterns.
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KEYWORD
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Listeria monocytogenes, Serovar, PFGE
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