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KMID : 1023520070300030385
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Korean Journal of Veterinary Service
2007 Volume.30 No. 3 p.385 ~ p.391
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Comparison of a new ELISA with other serodiagnostic tests for bovine brucellosis
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Hur Jin
Kakoma Ibulaimu
Jeong Jae-Myong
Lee Hyeon-Jin
Baek Byeong-Kirl
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Abstract
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A novel enzyme linked immunosorbent assay (ELISA) is described and compared with other established serologic tests for bovine brucellosis, namely the rose bengal test (RBT), the complement fixation test (CFT), and the tube agglutination test (TAT) approved and used in Korea. A total of 109 bovine serum samples were tested using all the 4 assays and analyzed as to specificity, sensitivity, reproducibility and predictive value. The ELISA showed 100% agreement with the CFT. The least agreement between ELISA was observed with the TAT. The agreement between the ELISA and RBT was not significantly different from that observed between the CFT and the ELISA. It is concluded that the new assay would be a good candidate for routine serologic survey for brucellosis in Korea. A protocol combining the ELISA and the CFT would increase the power for detection of serologically positive individuals and herds.
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KEYWORD
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Bovine brucellosis, ELISA, Complement fixation test, Rose bengal test, Tube agglutination test
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