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KMID : 1023520070300040505
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Korean Journal of Veterinary Service
2007 Volume.30 No. 4 p.505 ~ p.510
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Detection of beta-lactam antibiotic resistant genes in Escherichia coli from porcine fecal samples using DNA chip
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Park Nam-Yong
Na Sung-Ho
Cho Ho-Seong
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Abstract
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This study was conducted to detect -lactam antibiotic-resistant genes in the 400 E coli isolates from porcine fecal samples in Korea by a DNA chip. The DNA chip contains the specific probe DNAs of the -lactam antibiotic-resistant genes that had been labeled with a mixture of primer set designed to amplify specific genes (PSE, OXA, FOX, MEN, CMY, TEM, SHV, OXY and AmpC) using a multiplex polymerase chain reaction (PCR). Of 400 isolates 339 contained at least one -lactamases gene. Resistance to -lactamases was mediated mainly by AmpC (n = 339, 100%), and followed by TEM (n = 200, 59.0%), CMY (n = 101, 29.8%), PSE (n = 30, 8.9%) and both OXA and SHV genes (n = 20, 5.9%), while the FOX, MEN and OXY genes were not detected. The other sixty-one did not contain any -lactamase genes even though they were resistant to antimicrobial drugs. In conclusion, the DNA chip system can be used as a rapid and reliable method for detecting of -lactamases genes, which will help veterinarians select the antibiotics for monitoring and treating of animal diseases.
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KEYWORD
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Beta-lactam, Escherichia coli, DNA chip, Pig
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