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KMID : 1023520080310010087
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Korean Journal of Veterinary Service
2008 Volume.31 No. 1 p.87 ~ p.91
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Investigation of swine toxoplasmosis by Latex agglutination and polymerase chain reaction(PCR)
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Shim Hang-Sub
Choi Gyeong-Muk
Jeon Oh-Sook
Lee Su-Jin
Woo Jong-Tae
Ro Ki-Woan
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Abstract
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Between March and October 2007, a total of 516 blood samples from pigs in the Gyeonggi province were examined for seroprevalence against toxoplasmosis by latex agglutination test (LAT) and the detection of antigenic particles among seropositive samples by PCR. In the LAT, 118 (22.8%) were positive, and the unadjusted percentage of seroprevalence rates of breeding and fattening pigs were significant difference. Positive rate (14.1%) in the breeding pigs was much lower than that (27.8%) of the fattening pigs (p<0.001, Pearson’s Chi-square test). The antibody detection rate of sows was lower than fattening pigs, i.e., 15.8% (25/158) and 26% (93/358), respectively (P=0.011, Pearson’s Chi-square test). Among 118 seropositive samples by LAT, 68 (57.6%) were positive in PCR for the detection of the toxoplasma specific-DNA. There was a statistical difference in the positive PCR reaction between the raising pigs(63/93 67.7%) and sows (5/25, 20%) (P<0.01).
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KEYWORD
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Toxoplasmosis, Latex agglutination, PCR
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