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KMID : 1023520080310030385
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Korean Journal of Veterinary Service
2008 Volume.31 No. 3 p.385 ~ p.395
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A study on the residues of fluoroquinolones in hen’s eggs consumed in Incheon Metropolitan city
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Park Eun-Jeong
Lim Ji-Heun
Lee Sung-Mo
Im Jeong-Soo
Oh Dae-Kyu
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Abstract
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This study was carried out to investigate the residue level of fluoroquinolones in hen’s general eggs and specific eggs by microbiological assay method and high performance liquid chromatography (HPLC) method. HPLC separation was carried out by reversed phase chromatography on a Symmetry (2504.6 mm, particle size) with a phase composed of distilled water (containing 0.4% triethylamine and phosphoric acid) : Methanol (780 : 220, v/v), pumped isocratically at a flow rate of 1.0ml/min. A fluorescence detector was utilized with an excitation wavelength of 278nm and an emission wavelength of 456nm. The calibration curves were linear over a concentration range of . Average recoveries of the five fluoroquinolones in whole eggs at fortified levels of were ranged mean and low coefficient of variation was less than 10% for all analysed samples. The limits of detection and limits of quantification for whole eggs were and , respectively. Only one hen’s general eggfrom chicken farm in Incheon was detected with the residual fluoroquinolones (Microbiological assay method; 1 of 47 general eggs) ; the range of residual concentration enrofloxacin was 0.12ppm. Those in food stores were detected with the residual fluoroquinolones (Microbiological assay method; 4 of 88 general eggs) ; the ranges of residual concentration enrofloxacin were , ciprofloxacin , and hen’s specific eggs (40) in food stores were not detected. For the microbiological assay method of fluoroquinolones in hen’s eggs, as the results of comparative analysis, the disc diffusion method with E coli may be a little highly detected for the residual fluoroquinolones.
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KEYWORD
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fluoroquinolones, general egg, specific egg, Microbiological assay, HPLC
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