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KMID : 1023520090320010033
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Korean Journal of Veterinary Service
2009 Volume.32 No. 1 p.33 ~ p.41
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In vitro selection of lactic acid bacteria for probiotic use in pig
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Ryu Ji-Sook
Han Sun-Kyung
Shin Myeong-Su
Lee Wan-Kyu
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Abstract
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In order to develop probiotic strain for pigs, Lactobacillus spp. (527 isolates), Streptococcus spp. (95 isolates) and Bifidobacterium spp. (25 isolates) were isolated from the feces of 35 pigs. These isolates were tested through in vitro experiment such as acid tolerance at pH 2.0 (Lactobacillus spp. and Streptococcus spp.) or pH 3.0 (Bifidobacterium spp.), bile tolerance in MRS broth containing 0.3% (w/v) Oxgall, heat resistance at and for 5 min, antibiotic resistance, antimicrobial activity against pathogenic bacteria and Caco-2 cell adherence assay. Finally ten most superior strain (5 Lactobacillus spp. strain, 3 Bifidobacterium spp. strain and 2 Streptococcus spp. strain) were selected as potential candidate for probiotic use in pig industry. It could be used as an alternative to antibiotics in feed additives.
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KEYWORD
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Probiotics, Lactic acid bacteria, Acid and bile tolerance, Heat resistance, Antibiotic resistance, In vitro adherence assay
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