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KMID : 1023520090320020103
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Korean Journal of Veterinary Service
2009 Volume.32 No. 2 p.103 ~ p.109
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Detection of influenza A viruses by RT-PCR with single primer of nonstructural gene
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Moon Hyeong-sun
Bae Yoon-Yeong
Jin Ji-Dong
Kang Zheng-Wu
Hahn Tae-Wook
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Abstract
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Influeza type A virus have been worldwide problematic in animals as well as in humans. In this study, the use of reverse-transcriptase polymerase chain reaction (RT-PCR) was described for detecting influenza virus type A. The primer of RT-PCR was designed from an nonstructural (NS) gene of Influenza A virus. By RT-PCR, a product with the size of 189 bp was detected only when influenza virus type A was used as template. No products could be detected with Influenza virus type B as well as other respiratory pathogens. The detection limit of the RT-PCR was up to which is comparable to the sensitivity of cell culture method. The RT-PCR could detect the influenza A virus from nasal turbinates of the ferrets infected with influenza virus type A not type B.
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KEYWORD
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Influenza A viruses, RT-PCR, NS gene
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