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KMID : 1023520090320030257
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Korean Journal of Veterinary Service
2009 Volume.32 No. 3 p.257 ~ p.264
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Genotyping of Brucella abortus by PFGE
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Cho Min-Hee
Kim Seong-Guk
Kim Young-Hoan
Kim Soon-Tae
Eom Hyun-Jung
Jang Young-Sul
Ko Young-Hwal
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Abstract
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The process of subtyping is important epidemiologically for recognizing outbreaks of infection. Pulsedfield gel electrophoresis (PFGE) is considered the “gold standard” of molecular typing methods and a method of seperating large DNA molecules. In our study, Electrophoresis of DNA digested with the restriction endonuclease Xba I, Xho I and Smi I produced fragment profiles. Digestion of genomic DNA with Xba I and Xho I provided results that not distinguished among field strains of Brucella abortus which tested in study. But Smi I restriction enzyme resulted in two PFGE pattern consisting of 13-15 bands that ranged in size from 33 to 668 standard marker. Analysing DNA fingeprinting software, cluster analysis showed 93.75% similar in two PFGE pattern.
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KEYWORD
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Brucella abortus, Molecular typing, PFGE
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