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KMID : 1023520090320040299
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Korean Journal of Veterinary Service
2009 Volume.32 No. 4 p.299 ~ p.306
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Rapid detection and quantification of porcine circovirus type 2 (PCV 2) DNA in Real-time PCR
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Kim Eun-Gyeong
Hwang Bo-Won
Lee Jong-Min
Son Byeong-Guk
Park Ho-Jung
Kim Do-Kyoung
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Abstract
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Assay for the detection and quantification of porcine circovirus type 2 (PCV 2) with the real-time PCR were developed. TaqMan probe real-time using a set of primer/probe was developed for detection of PCV 2. In this study we applied real-time PCR assay to 320 samples, collected from pig farms. In 151 of 320 samples, PCV 2 DNA was detected by conventional PCR assay. All samples positive for PCV 2 DNA in conventional PCR assay were also positive in Real-time PCR assay, but 69 of 169 samples that tested negative for PCV 2 DNA in conventional assay were tested positive in TaqMan probe real-time PCR assay. The test of TaqMan probe real-time PCR resulted in detection and quantification limits of 101 copies per sample. TaqMan probe real-time PCR assay increased the number of samples in which PCV 2 was detected by 21%. TaqMan probe real-time PCR assay is very efficient method in contrast to the conventinal PCR, becoming increasingly important method for gene analysis.
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KEYWORD
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Porcine circovirus type 2, TaqMan probe real-time PCR
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