|
KMID : 1023520110340030201
|
|
Korean Journal of Veterinary Service
2011 Volume.34 No. 3 p.201 ~ p.208
|
|
|
The biochemical and molecular characteristics of Streptococcus equi subsp. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea
|
|
Choi Seong-Kyoon
Kim Seong-Guk
Cho Gil-Jae
|
|
|
Abstract
|
|
|
|
Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is a pathogen of a variety of infections in horse. We studied biochemical and molecular characteristics of S. zooepidemicus isolated from the genital tract of Thoroughbred mares in Korea. Seventy-nine isolates were identified as S. zooepidemicus by biochemical and PCR method from 374 horses. The biochemical characteristics of S. zooepidemicus isolates were positive reaction of lactose and sorbitol. However, S. zooepidemicus isoltes were negative reaction of inulin, mannitol, raffinose, trehalose, aesculin hydrolysis, growth in 6.5% NaCl and variable reaction of maltose. Epidemiological investigations of S. zooepidemicus isolates were performed by fragment analysis of SzP (S. zooepidemicus protective protein) gene, CNE (collagen binding protein) gene and ISR (16s rRNA intergenic spacer region) gene using ABI Prism 3,130 ×l Genetic Analyzer System. All isolates were shown single amplification size of 906 bp in CNE gene, but SzP and ISR gene were shown variable patterns of fragment size. The characteristics of S. zooepidemicus investigated in this study will be very useful for the prevention of infection and the studies of epidemiologic characteristics of S. zooepidemicus, causing the severe economic losses due to reproductive failures.
|
|
|
KEYWORD
|
|
|
Thoroughbred horse, Genital tract, Streptococcus equi subsp. zooepidemicus
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|