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KMID : 1023520110340040341
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Korean Journal of Veterinary Service
2011 Volume.34 No. 4 p.341 ~ p.352
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Evaluation of bacteriophages for prevention and treatment of diarrhea due to experimental enterotoxigenic Escherichia coli K88 infection of weaned piglets
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Kim Sung-Jae
Lee Chang-Hee
Yang Don-Sik
Han Jeong-Hee
Kim Nam-Hee
Kim Jin-Dong
Yang Si-Yong
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Abstract
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Colibacillosis in pigs remain a major swine industry bruden worldwide. Although some progress has been made in treating collibacillosis in pigs by using biosecurity and antimicrobials, it still remain a considerable problem. The use of host-specific bateriophages as a biocontrol is one possible alternative. The purpose of this study was to evaluate the effect of bacteriophage against enterotoxigenic Escherichia coli (ETEC) K88 infection in piglets. Twenty-eight piglets were randomly divided into four groups and each group was allocated with 7 pigs. Group B, C and D were inoculated with 5 ml of ETEC K88 (1×108 CFU/ml) per head of piglet via oral. Group C and D were fed with bacteriophages (Group C, 1.0×106 PFU/g; Group D, 1.0×108 PFU/g; CJ CheilJedang Corp., Korea) orally as treatment. In piglets administrated bacteriophages and challenged with ETEC K88 (Group C and D), Clinical signs and the growth performance were improved and antibody titers were maintained low level compared with piglets challenged with ETEC K88 (Group B, P＜0.05). Group B were shown high pH in the alimentary tracts compared with other piglets (P＜0.05). In quantitative analysis by real-time PCR, the results of Group C and D were lower than those Group B in faecal and intestinal samples (P＜0.05). Severe villus atrophy and crypt hyperplasia were observed in Group B consequently V/C ratio increased, compared with other piglets. These results indicate that feeding with bacteriophage has effect to prevent ETEC K88 infection in piglets and suggest that use of bacteriophage can be considered a valid antibiotic alternative.
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KEYWORD
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Bacteriophage, Enterotoxigenic Escherichia coli (ETEC), Quantitative real-time PCR, Gastrointestinal pH, V/C ratio
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