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KMID : 1023520120350040283
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Korean Journal of Veterinary Service
2012 Volume.35 No. 4 p.283 ~ p.288
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PFGE patterns of Streptococcus suis isolates from diseased pigs in Gyeongbuk province, Korea
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Kim Seong-Guk
Kim Young-Hoan
Lee Hong-Young
Yun Mun-Jo
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Abstract
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Streptococcus(S.) suis is a pathogen, causing meningitis, septicemia and sudden death in weaning piglets as well as fattening pigs. Using multiplex PCR method based S. suis capsular genes, 61 S. suis isolates was classified as serotypes 2, 7, 9 and untypable. Genotyping of S. suis isolates was analysed by PFGE pattern with treated Sma I restricted enzyme. Of the 61 S. suis, 25 (40.9%) were serotype 2, 6 (9.8%) were serotype 7, 5 (8.2%) were serotype 9, and 25 (40.9%) were untypable, respectively. Twenty four PFGE patterns were detected in this study and also PFGE patterns were classified according to serotype; serotype 2 was classified as 6 genotypes, serotype 7 was 5 genotypes, serotype 9 was 3 genotypes, and untypable was 11 genotypes, respectively.
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KEYWORD
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Streptococcus suis, Multiplex PCR, Serotype, PFGE, Genotype
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