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KMID : 1023520120350040289
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Korean Journal of Veterinary Service
2012 Volume.35 No. 4 p.289 ~ p.294
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A newly developed consensus polymerase chain reaction to detect Mycoplasma species using 16S ribosomal RNA gene
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Hong Sun-Hwa
Park Sang-Ho
Chung Yung-Ho
Kim Ok-Jin
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Abstract
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Mycoplasmas are highly fastidious bacteria, difficult to culture and slow growing. Infections with Mycoplasma species can cause a variety of problems in living organisms and in vitro cell cultures. In this study, we investigated the usefulness of a genus-specific consensus PCR analysis method to detect Mycoplasma species. The developed consensus primer pairs MycoF and MycoR were designed specifi-cally to amplify the 16S ribosomal RNA gene (rRNA) of Mycoplasma species by the optimized PCR system. The developed consensus PCR system effectively amplified 215 bp of Mycoplasma ge-nus- specific region of 16S rRNA. In conclusion, we recommend this consensus PCR for monitoring Mycoplasma species in animals, human and cell culture system.
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KEYWORD
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Mycoplasma, PCR, Consensus, 16S rRNA, Genus-specific
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