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KMID : 1023520120350040333
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Korean Journal of Veterinary Service
2012 Volume.35 No. 4 p.333 ~ p.337
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Investigation of bovine leukemia virus infection in dairy farms of northern Gyeonggi province, Korea
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Jung Kwang
Shim Hang-Sub
Baek Jin-Joo
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Abstract
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This study was carried out to investigate the prevalence of bovine leukemia virus (BLV) infection and to compare the results of enzyme-linked immunosorbent assay (ELISA) and nested polymerase chain reaction (nPCR) in dairy farms in northern Gyeonggi province from August through December 2011. A total of 625 dairy cattle from 14 dairy farms were tested for antibodies against BLV using commer-cially available ELISA test kit. The overall seroprevalence of BLV infection was 76.3%. The seropreva-lence of diary cattle according to age was the highest at 61∼72 months (88.0%, P<0.001). Two hun-dred fifty one dairy cattle from 7 diary farms were tested ELISA and nPCR. The kappa value of BLV between ELISA and nPCR was 0.765. The results indicate that BLV infection spread widely in dairy farms and the nPCR is rapid method for the early detection of BLV infection.
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KEYWORD
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Bovine leukemia virus (BLV), Dairy cattle, ELISA, PCR
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