KMID : 1023520130360020073
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Korean Journal of Veterinary Service 2013 Volume.36 No. 2 p.73 ~ p.78
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Analysis of the antibiotic resistance gene in Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province
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Kim Joo-Hyung
Kim Seon-Soo Kim Seong-Guk Kim Jung-Hwa Park Sye-Hee Nam Ki-Hu Kim Hyoung-Bae
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Abstract
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This study was conducted to investigate antibiotic resistance among Salmonella Typhimurium isolates from diseased pigs in Gyeongbuk province during the period 1998¡2011. One hundred forty one isolates were tested for antibiotic resistance using the standard disk diffusion method and were examined for presence of resistance gene by PCR method. S. Typhimurium showed high drug resistance rates to tetracycline (95.7%), streptomycin (93.6%), ampicillin (86.5%), cephalothin (80.1%), gentamicin (79.4%), and trimethoprim/sulfamethoxazole (72.3%). Resistance gene, blaTEM, blaPSE1, tetA, tetB, tetG, sul1, sul2, aadA, strA, grm, and temA were detected among the antibiotic resistance isolates and temB, tetC, aadB gene were not detected. One hundred twenty one (89.6%) tetA, two (1.5%) tetB and one (0.7%) tetG gene were detected in the 135 tetracycline resistant isolates. Two (1.6%) temA gene were detected in one hundred twenty two ampicillin resistance isolates and temB was not detected.
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KEYWORD
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Salmonella Typhimurium, Resistance gene, PCR, Antibiotic
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