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KMID : 1023520130360030181
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Korean Journal of Veterinary Service
2013 Volume.36 No. 3 p.181 ~ p.186
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Isolation, identification and serological investigation of Actinobacillus pleuropneumoniae in slaughtered pigs
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Kim Kyung-Eon
Ku Kyung-Nyer
Ko Jae-Hyung
Moon Hyeong-Jun
Choi Kwon-Rag
Song Eun-Ah
Park Mi-Young
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Abstract
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This study was conducted to isolate the Actinobacillus pleuropneumoniae (APP) and to find out the distribution of 15 serovars mainly in southern Gyeonggi province, Korea. From July 2011 to Nov. 2012, a total of 2,204 slaughter pigs (110 herds) were inspected for evaluation of APP like pneumonic lesions. 48 (33.8%) APP strains were isolated from the 142 lungs and identified using PCR assays (cps, apx/omlA, biovar). Consequently, the serotype ratio were as in the following; type2 41.7% (n=20), type5 33.3% (n=16), type12 10.4% (n=5), type1 6.2% (n=3), type4 and 7 2.1% (n=1) and unknown 4.2% (n=2). Also serological test was implemented for 452 (83 herds) serum samples randomly collected from above slaughter pigs using commercial ELISA kits. The positive ratio of each serotype for tested pigs were 19.1% (77/404) on [2], 7.1% (32/452) on [3, 6, 8], 6.9% (28/404) on [5a, 5b], 6.2% (28/452) on [4, 7], 2.8% (9/320) on [12], 2.0% (9/452) on [1, 9, 11] and 0.0% (0/452) on [10]. And 49.3% (223/452) of pigs were positive on apxⅣ antibody. On the basis of latter screening test, the infected farm ratio accounted for 71.1% (59/83) and that was much higher than previously reported data.
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KEYWORD
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Actinobacillus pleuropneumoniae, Slaughter pigs, PCR, ELISA
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