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KMID : 1023520140370020123
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Korean Journal of Veterinary Service
2014 Volume.37 No. 2 p.123 ~ p.129
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Origin and effective ingredient standards of honeybee venom as natural antibiotic ingredients
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Han Sang-Mi
Kim Jung-Min
Yeo Joo-Hong
Hong In-Pyo
Woo Soon-Ok
Lee Kwang-Gill
Kweon Hae-Yong
Han Sang-Hoon
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Abstract
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This research was performed in order to investigate the origin, standard compound, and structural and physical properties of honeybee venom which used as natural antibiotic ingredients to animal. We compared the nucleotide sequence of mitochondrial cytochrome c oxidase subunit 1 gene (COI) of honeybees were collected from Gangwon, Gyeonggi, Chungnam, Gyeongbuk, Gyeongnam province and Suwon. As major constituent of honeybee venom, melittin was assayed by liquid chromatography. X-ray, differential scanning calorimetry (DSC) and fourier transform infrared spectroscopy (FT-IR) were utilized to examine the structural and physical properties of honeybee venom. Based on the 627bp sequence of COI, Apis mellifera ligustica was determinated honeybees collected from all six regions. Melittin content varied from 50.7 to 68.6 and averaged 59.8%. According to XRD analysis, honeybee venom showed regular crystal structure peaks at 2θ=8.5° and 21.5°. DSC showed that the maximum degration temperature of powder was around 230°C. Through FT-IR analysis, we could identify cross-linking by the presence of peptide peak at 1,500∼1,600 cm-1. In conclusion, the origin of honeybee venom was Apis mellifera ligustica and effective ingredient standards was melittin content varied from 50.7 to 68.6 as natural antibiotic ingredients.
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KEYWORD
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Honeybee venom, Apis mellifera ligustica, Melittin, Natural anitbacterial agents
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