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KMID : 1023520140370030151
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Korean Journal of Veterinary Service
2014 Volume.37 No. 3 p.151 ~ p.156
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Comparative evaluation of two commercial ELISA kits for detection of PRRS antibodies using sera collected from pigs in various stages of PRRSV infection
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Seo Byoung-Joo
Kim Won-Il
Kim Hyoun-Il
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Abstract
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Porcine reproductive and respiratory syndrome virus (PRRSV) causes major economic losses to the Korean pig industry. ELISA tests using recombinant nucleocapsid protein of PRRSV have been most commonly used for PRRS diagnostics. In the current study, two commercial PRRSV ELISA kits (Bionote PRRSV Antibody ELISA and IDEXX 3XR PRRS Antibody ELISA) have been compared using sera collected from 19 swine farms in various stages of PRRSV infection confirmed by professional diagnostic centers. Thus 130 sera collected from 5 different farms with active PRRSV infection, 130 sera from 6 different farms with PRRS-stabilized status, and 140 sera from 8 different farms with PRRS-free status were evaluated to determine the correlation of test results between those ELISA kits. Both ELISA kits showed a good correlation [PRRSV-positive farms (R2=0.6375) and stabilized farms (R2=0.8928)] in sample-to-positive (S/P) ratio values. Among the 140 sera from negative farms, one sample was falsely positive by either of the ELISA kits. In conclusion, both of the ELISA kits showed a good correlation when applied on field samples collected from farms at various stages of PRRSV infection. Bionote ELISA or IDEXX ELISA gave a false positive result on 1 out of 140 negative samples so their specificity was calculated as 99.3%. Therefore, Bionote ELISA would be a good complementary and alternative method for IDEXX ELISA kit, and vice versa.
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KEYWORD
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PRRSV, ELISA, Antibody, Evaluation, Field application
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