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KMID : 1023520150380010025
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Korean Journal of Veterinary Service
2015 Volume.38 No. 1 p.25 ~ p.30
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Analysis of bee venom residues in milks of dairy cattle using UHPLC with newly developed pre-processing method
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Han Sang-Mi
Hong In-Pyo
Woo Soon-Ok
Kim Se-Gun
Jang Hye-Ri
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Abstract
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Bee venom has been used as to prevent and treat bovine mastitis as natural antimicrobial compounds in some dairy cattle farms in Korea. It is needed to determine the residual of bee venom in milks of dairy cattle treated with bee venom. Since bee venom is not approved as a raw material for animal drugs, the preprocessing method to detect bee venom residual in milk and the tolerance limit for its residue has not been established yet in Korea. Therefore, the purpose of this study was to develop pre-processing method not affecting major component of bee venom for detection of its residue in milks using ultra-high performance liauid chromatography (UHPLC). In addition, bee venom residue was also analyzed in milk samples of dairy cattle treated for mastitis with bee venom using UHPLC with the developed pre-processing method in this study. As a result, melittin, histamin and phospolipase A2, the major components of bee venom, were all detected by UHPLC with the pre-processing method developed in this study. The results of this study suggest that the pre-processing method developed in this study can be useful to detect bee venom residue in dairy cattle milk. We also found that no bee venom residues were detected in milk samples collected from dairy cattle treated with bee venom after 1 and 3 days, respectively.
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KEYWORD
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Honeybee venom, Residues, Milks, Dairy cow, Pre-processing method
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