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KMID : 1023520150380020101
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Korean Journal of Veterinary Service
2015 Volume.38 No. 2 p.101 ~ p.106
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A 16S rDNA polymerase chain reaction assay to detect Mycoplasma pulmonis in rats model
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Hong Sun-Hwa
Lee Hyun-A
Kim Ok-Jin
Choi Yeon-Shik
Chung Yung-Ho
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Abstract
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Murine mycoplasmosis, caused by Mycoplasma (M.) pulmonis, is a prominent disease in rodent animals. The aim of this study was to develop a sensitive and specific PCR assay to detect M. pulmonis in animals and to assess the suitability of this assay for the detection of mycoplasmal infection in rats experimentally infected with M. pulmonis. A new PCR assay using the M. pulmonis-specific primer pairs MPul-F and MPul-R was developed. The primers and probe for the assay were designed from regions in the 16S rRNA gene that are unique to M. pulmonis. The novel PCR assay was very specific and sensitive for M. pulmonis, detecting the equivalent of 5 pg of target template DNA. It detected only M. pulmonis and no other Mycoplasma species or other bacterial species. The newly developed PCR assay also effectively detected M. pulmonis infection in rats. These results suggest that this PCR assay using M. pulmonis-specific primer pairs of MPul-F and MPul-R will be useful and effective for monitoring M. pulmonis infection in animals.
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KEYWORD
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Mycoplasma, Mycoplasma pulmonis, 16S rRNA, Pneumonia, Rat
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