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KMID : 1023520150380020107
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Korean Journal of Veterinary Service
2015 Volume.38 No. 2 p.107 ~ p.116
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Loop-mediated isothermal amplification assay for the rapid detection of swine influenza virus
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Kim Eun-Mi
Kim Hee-Jung
Yeo Sang-Geon
Park Choi-Kyu
Jeon Hyo-Sung
Kim Ji-Jung
Shin Yeun-Kyung
Song Jae-Young
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Abstract
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In this study, we developed a rapid, sensitive and specific reverse-transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detection of swine influenza viruse (SIV) including major subtypes of swine influenza viruses H1N1, H1N2 and H3N2, and a novel subtype of influenza A virus that accidentally infected in pig population. The RT-LAMP was completed in 40 min at 58oC and the sensitivity of the RT-LAMP (1 copy/ L) was 10-fold higher than conventional reverse transcription-polymerase chain reaction (RT-PCR) (10 copy/ L) and the same to real time RT-PCR (1 copy/ L). Also, the result of the RT-LAMP can be confirmed without any detection system. Therefore, the RT-LAMP could be a alternative diagnostic method for SIV detection in national SIV monitoring system and clinical diagnostic laboratory in the future.
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KEYWORD
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Swine influenza virus, SIV, Loop-mediated isothermal amplification, LAMP, Reverse transcription-polymerase chain reaction, Real time RT-PCR, rRT-PCR
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