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KMID : 1023520150380020141
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Korean Journal of Veterinary Service
2015 Volume.38 No. 2 p.141 ~ p.144
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The first virus isolation and partial characterization of equine herpesvirus-4 in a horse, South Korea
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Choi Eun-Jin
Lee Hyun-Kyoung
Lee Kyoung-Hyun
So Byoung-Jae
Song Jae-Young
Do Jae-Chul
Yang Seon-Joo
Lee Hyun-Chul
Yang Young-Jin
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Abstract
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An equine herpesvirus-4 (EHV-4) was isolated in nasal swabs collected in a horse showing respiratory clinical signs. Equine dermis cells inoculated with the sample were observed with characteristic viral cytopathic effects after 3 days of postinoculation and the infected cells exhibited bright intracelluar fluorescence by indirect immunofluorescence assay. At the nucleotide level, the partial glycoprotein B gene of the Korean EHV-4 isolate (K001) had 99.9% identity to 1942 strain (GenBank No. M26171). To author’s knowledge, the report describes the first isolation and partial characterization of EHV-4 in Korea. The virus can be used for further study of EHV-4.
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KEYWORD
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Equine herpesvirus-4, Korea, Virus isolation, Immunofluorescence assay, Glycoprotein B
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