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KMID : 1023520160390030159
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Korean Journal of Veterinary Service
2016 Volume.39 No. 3 p.159 ~ p.166
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Antibacterial effects of purified bee venom against some pathogenic bacteria isolated from dead chickens
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Han Sang-Mi
Kim Se-Gun
Hong In-Phyo
Woo Soon-Ok
Jang Hye-Ri
Lee Kyung-Woo
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Abstract
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Clostridium perfringens, Salmonella thyphimurium and S. Montevideo isolated from the intestines of dead broiler chickens in Korea were tested for antibacterial effects to purifed bee venom. Purified bee venom from Apis mellifera L. has been used as natural antimicrobial compounds in pigs, cows, dairy cattle and chicken farms in Korea. To investigate antibacterial effect of purified bee venom was eval-uated by agar well diffusion method, minimum inhibitory concentraion (MIC), minimum bactericidal concentration (MBC), and postantibiotic effect (PAE). Purified bee venom exhibited significant in-hibition of bacterial growth of C. perfringens, S. thyphimurium and S. Montevideo with MIC value of 0.85, 0.68 and 0.69 μg/mL, respectively. The MBC value of purified bee venom against C. perfringens, S. thyphimurium and S. Montevideo were 3.33, 2.66 and 2.86 μg/mL. Furthermore, the results of PAE values against C. perfringens, S. thyphimurium and S. Montevideo showed the bacterial effect with 3.5, 4.0 and 3.5 hr. Stability of pufifed bee venom at acidity from pH 1 to pH 8 for 24 hr was the anti-bacterial activity for C. perfringens, S. thyphimurium and S. Montevideo and melittin contents. Also pu-rified bee venom processed through the heating for 15 min, there was no signification loss of the anti-bacterial activity and melittin at below 100 ℃. These results obtained in this study suggest that purified bee venom might be utilized as a feed additive in poultry diets.
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KEYWORD
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Bee venom, Necrotic enteritis, Fowl typhoid, Chicken, Antibacterial
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