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KMID : 1023520160390040247
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Korean Journal of Veterinary Service
2016 Volume.39 No. 4 p.247 ~ p.251
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MALDI TOF MS for the identification of Salmonella spp. from swine
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Sohn Jun-Hyung
Jeon Woo-Jin
Lee Young-Mi
Kim Seon-Soo
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Abstract
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Salmonella is one of the most common bacteria that causes heavy losses in swine industry and major causative pathogen of food poisoning in public health. Various methods for the identification of Salmonella such as Gram staining, agglutination test, enzyme-linked immunosorbent assay (ELISA), pol-ymerase chain reaction (PCR) have been used. Several studies have demonstrated that Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI TOF) Mass Spectrometry (MS) identification is an efficient and inexpensive method for the rapid and routine identification of isolated bacteria. In this study, MALDI TOF MS could provide rapid, accurate identification of Salmonella spp. from swine compared with end point PCR and real time PCR.
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KEYWORD
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Idendification, MALDI TOF MS, End point PCR, Real time PCR, Salmonella
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