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KMID : 1023520160390040259
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Korean Journal of Veterinary Service
2016 Volume.39 No. 4 p.259 ~ p.266
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Genetic sequence and phylogenetic analysis of spike genes of Porcine epidemic diarrhea virus (PEDV) in Chung-Nam area
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Park Hyo-Sun
Yook Sim-Yong
Jeon Dong-Min
Lee Jin-Ju
Sin Chang-Ho
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Abstract
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Porcine epidemic diarrhea virus (PEDV) causes an acute and lethal watery diarrhea in piglets that is great economic losses to the swine country worldwide. The spike (S) glycoprotein is an important determinant for PEDV biological properties. In the present study, we determined the full-length S gene sequences of five Chung-nam PEDV field isolates collected in 2016. The S gene was amplified by RT-PCR, purificated, sequenced, analyzed and then compared with published sequences of other PEDV strains. 5 field strains share 98.5%∼99.9% homologies with each other at the nucleotide sequence level and 96.7%∼99.9% homologies with each other at the amino acids sequence level. Most field strains have nucleotide insertions, deletions and mutation regions, and show lower homologies (93.1∼93.8%) with classical and vaccine strains, however higher homologies (99.1%∼99.5%) with US PEDV isolates in 2013. By phylogenetic tree analysis based on nucleotide sequence, five PEDV field isolates were clustered into Genogroup 2b but differ genetically from the vaccine strains (SM-98 and DR-13).
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KEYWORD
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PEDV, S gene, PCR, Phylogenetic analysis
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