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KMID : 1023520170400010001
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Korean Journal of Veterinary Service
2017 Volume.40 No. 1 p.1 ~ p.6
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Random amplified polymorphic DNA analysis of bacterial pathogens using universal rice primers
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Monoldorova Sezim
Kim Jin-Sol
Kim Joon-Hee
Jeon Bo-young
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Abstract
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Molecular typing of pathogenic microorganisms is important for epidemiological investigation of infectious disease outbreaks. In this study, we applied Universal Rice Primers (URP) that were originated from repetitive sequences in rice chromosomal DNA to random amplified polymorphic DNA (RAPD) analysis of pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, and Salmonella sp. Of the twelve URP primers examined to date, seven primers (URP-2, -3, -4, -5, -6, -8, and -9) generated reproducible and polymorphic PCR products ranging from 1 to 13 bands. One of them, URP-6 was very effective in differentiating seven E. coli serotypes, seven L. monocytogenes clinical isolates, and eight Salmonella subspecies (ssp.) serovars. The results thus indicate that RAPD analysis using URP primers might be useful in typing bacterial pathogens including E. coli, L. monocytogenes, and Salmonella strains.
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KEYWORD
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RAPD analysis, URP primers, E. coli, Listeria monocytogenes, Salmonella
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