KMID : 1023520180410030171
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Korean Journal of Veterinary Service 2018 Volume.41 No. 3 p.171 ~ p.177
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Stimulation of the milk protein production in MAC-T cells by purified bee venom
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Han Sang-Mi
Woo Soon-Ok Kim Se-Gun Jang Hye-Ri
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Abstract
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Purified bee venom was collected from colonies of honeybees (Apis mellifera L.) using a bee venom collector under sterile conditions and then purified under strict laboratory conditions. Purified bee venom contained 63.9¡¾5.4% melittin, 10.9¡¾1.6% phospholipase A2, and 2.3¡¾0.3% apamin. Purified bee venom has various anti-bacterial, anti-inflammatory and immunostimulating effects. In this study, we evaluated purified bee venom which are mammary gland cells, MAC-T cells are used to increase the synthesis of milk protein. Purified bee venom promoted the proliferation of MAC-T cells at concentrations below 1 ¥ìg/mL, but cytotoxicity at 10 ¥ìg/mL and above. As a result of the increase in the synthesis of ¥â-casein, a milk protein after treatment with MAC-T cells at a concentration of the bee venom without cytotoxicity, the ¥â-casein content in the cell culture was increased when treated at a concentration of 1 ng/mL or more. In addition, it was confirmed that purified bee venom significantly increased the expression of bovine ¥â-casein (bCSNB) mRNA, a ¥â-casein synthesis gene, at a concentration of 1 ng/mL or more. These results suggest that purified bee venom can be used to increase the production of livestock by ultimately increasing the expression of milk protein
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KEYWORD
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Apis mellifera L., Purified bee venom, MAC-T cells, Casein, bCSNB
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