|
KMID : 1023520180410030197
|
|
Korean Journal of Veterinary Service
2018 Volume.41 No. 3 p.197 ~ p.202
|
|
|
Comparison of histopathological and molecular diagnostic methods in Helicobacter suis infection in Korea
|
|
Lee Jae-Ha
Lee Sun-Gue
Han Jeong-Hee
|
|
|
Abstract
|
|
|
|
Helicobacter suis is a gram negative bacterium and colonizes in porcine stomach. It causes gastric diseases in the stomach and plays a significant role in daily weight gains in pigs. Recent studies about one of potential sources of human gastric diseases. Therefore, this study was conducted to compare histopathological lesions and molecular detection of Helicobacter suis in the pyloric mucosa of porcine stomachs transferred from slaughterhouses, based on gross and histological examinations and a PCR assay. A total 90 stomach samples were investigated to record gastric lesion scores by characteristic gastric lesions, followed by routine H & E and Warthin-Starry silver staining to detect Helicobacter-like organisms. For PCR assay, H. suis specific primers and conditions are used. Sixty-one samples (67.8%) showed gross gastric lesions, of which 38 samples (40.2%) presented grade 1, 12 samples (13.3%) presented grade 2, and 11 samples (12.2%) presented grade 3, respectively. In Warthin-Starry silver stain, Helicobacter-like organisms were detected from 11 samples (12.2%) with 4 samples (4.4%) for grade 0, 5 samples (5.6%) for grade 1, 1 sample (1.1%) for grade 2 and 1 sample (1.1%) for grade 3, respectively. The PCR resulted positive in 37 samples (41.1%) with 14 samples (15.6%) for grade 0, 14 samples (15.6%) for grade 1, 3 samples (3.3%) for grade 2 and 6 samples (6.7%) for grade 3, respectively. Positive samples for both examinations were 5 samples (5.6%). The result suggested that it should be considered as one of factors causing a gastric disease in pigs. Also, it could be acknowledged to research fundamental aspects of Helicobacter-induced gastritis in human as an animal model.
|
|
|
KEYWORD
|
|
|
Helicobacter suis, Gastric gross lesion, Warthin-Starry silver stain, PCR
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|