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KMID : 1023520190420010017
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Korean Journal of Veterinary Service
2019 Volume.42 No. 1 p.17 ~ p.24
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Characterization of plasmid-mediated quinolone resistance genes in Enterobacteriaceae isolated from companion animals
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Cho Jae-Keun
Kim Jeong-Mi
Kim Hwan-Deuk
Kim Kyung-Hee
Lim Hyun-Suk
Yang Chang-Ryoul
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Abstract
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The aim of this study was to investigate the prevalence and characterization of plasmid-mediated quino-lone resistance (PMQR) gene in 79 Enterobacteriaceae isolated from dogs and cats. Of 79 isolates, PMQR genes were found in 10 (12.7%) isolates, including aac(6’)-lb-cr, qnrB, qnrS and qnrA detected alone or in combination in 8 (10.1%), 4 (5.1%), 2 (2.5%) and 1 (1.3%) isolates, respectively. Interest-ingly, two qnrS genes were detected in nalidixic acid and ciprofloxacin susceptible isolates. Extended- spectrum ?-lactamase (ESBL) was detected in 90% (9 isolates) of PMQR positives isolates. Among ESBL genes, CTX-M, TEM and SHV were detected in 9, 8 and 3 isolates, respectively. Almost all PMQR genes were detected in co-existence with ESBL genes. All PMQR positives isolates were multi-drug resistance (i.e. resistant to five or more antibiotics). qepA, OXA and CMY-2 genes were not found. The six transconjugants were obtained by conjugation experiment. The aac(6’)-lb-cr, qnrB and qnrS were co-transferred with CTX-M, TEM and/or SHV, whereas qnrA was not observed among transcon-ugants. This is the first report of the presence of aac(6’)-lb-cr and qnrA gene among Enterobacteriaceae isolates from dogs in Korea. The prudent use of antimicrobials and continuous monitoring for com-panion animals are required.
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KEYWORD
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Enterobacteriacea, Dogs, PMQR, ESBL, Transconjugants
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