|
KMID : 1023520190420030135
|
|
Korean Journal of Veterinary Service
2019 Volume.42 No. 3 p.135 ~ p.144
|
|
|
Pathology and virus distribution in the lymphoid tissues of chicks co-infection with H9N2 Avian influenza and Newcastle disease virus
|
|
Le Sung-Min
Cho Eun-Sang
Choi Hwan-Won
Choi Bo-Hyun
Son Hwa-Young
|
|
|
Abstract
|
|
|
|
Low pathogenic avian influenza (LPAI; H9N2) and Newcastle disease (ND) are economically important poultry diseases in Korea. In this study, we investigated pathological features and virus distribution in the lymphoid tissues of chicks experimentally infected with H9N2 and/or ND virus. Six-weeks-old SPF chickens were divided into 4 groups, Control (C), H9N2 (E1), NDV (E2), and H9N2+NDV (E3). E1 group was challenged with 0.1 ml A/Kr/Ck/01310/01 (H9N2) 105.6 EID50 intranasally, E2 group was challenged with 0.5 ml KJW (NDV) 105.0∼106.0 ELD50 intramuscularly, and E3 group was challenged with H9N2, followed 7 days later by NDV. In histopathological examination, E1 group showed depletion and necrosis in bursa of Fabricius, thymus, cecal tonsil, and spleen, whereas E2 and E3 groups were noted severe lymphocyte depletion and necrosis with destruction of lymphoid organs structures. In TUNEL assay, apoptotic bodies were detected in lymphoid organs of all experimental groups, which was most severe in E3 group. H9N2 and ND viruses were predominantly detected in cecal tonsil of E1, E2, and E3 groups by PCR and immunohistochemistry (ICH). In conclusion, co-infection of H9N2 with NDV caused severe pathologic lesions and apoptosis in lymphoid tissues compared to single infections.
|
|
|
KEYWORD
|
|
|
SPF chicken, LPAI, ND, Lymphoid tissues, Pathology
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|