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KMID : 1023520190420030153
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Korean Journal of Veterinary Service
2019 Volume.42 No. 3 p.153 ~ p.159
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Mutation in gyrA gene of nalidixic acid-resistant Salmonella isolates isolated from poultry slaughterhouse
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Cho Jae-Keun
Son Kyu-Hee
Kim Kyung-Hee
Kim Jeong-Mi
Park Dae-Hyun
Lee Jung-Woo
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Abstract
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The objective of this study was to identify mutations in the quinolone resistance determining region (QRDR) of the gyrA, gyrB, parC and parE genes, and the presence of plasmid-mediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrS, aac(6’)-lb-cr and qepA in 40 nalidixic acid- resistant (NAR) Salmonella isolates isolated from poultry slaughterhouse. The MIC of NA and ciprofloxacin for 40 NAR Salmonella isolates was 128∼512 μg/mL and ＜0.125∼0.25 μg/mL, respectively. The Salmonella isolates were resistant to NA (100%), gentamicin (5.0%) and ampicillin (2.5%). All NAR Salmonella isolates represented point mutation in codons Aspartic acid(Asp)-87 (90%) and Serine(Ser)-83 (10%) of QRDR of gyrA gene: Asp87→glycine, Ser83→tyrosine. No mutations were observed in QRDR of the gyrB, parC and parE gene. Moreover PMQR genes was not found in any of the tested isolates. Our findings showed that DNA gyrase is the primary target of quinolone resistance and a single mutation in codon Asp87 and Ser83 of the gyrA gene can confer resistance to NA and reduced susceptibility ciprofloxacin in Salmonella isolates.
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KEYWORD
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Salmonella, gyrA, Nalidixic acid, Ciprofloxacin, PMQR
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