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KMID : 1023520200430030147
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Korean Journal of Veterinary Service
2020 Volume.43 No. 3 p.147 ~ p.154
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Development and evaluation of standard samples for quality control of automated total bacterial counter in raw milk
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Kang Hye-Jeong
Kim Jin-Hwan
Byun Yeong-Seob
Lee Ha-Na
Lee Hye-Young
Kim Ji-Hyeon
Hong Se-Rim
Kim Ha-Young
Yoon Soon-Seek
Moon Jin-san
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Abstract
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Standard samples were prepared for this study, and applied for BactoScan TM and BactoCount TM in order for quality control evaluation for total bacterial count in raw milk. Accordingly, the preparation of two lots of standard samples for quality control were lyophilized, which contain Lactobacillus lactis. The standard samples were prepared into three different levels of bacterial counts, those were Low 30,000∼ 40,000, Medium 70,000∼90,000, High 150,000∼220,000 CFU/mL, respectively. Then, the proficiency tests were performed in total 19 laboratories for measuring total bacterial counts. The total bacterial counts in the standard samples showed 37∼42, 82∼105, 214∼240 CFU/mL in the first lot, and it showed 30∼36, 67∼75, 131∼163 CFU/mL in the second lot in low, medium and high levels, respectively. Based on these results, the absolute values of z-scores of six standard samples in 18 laboratories were ≤2, which means the samples are satisfactory. However, z-score in one laboratory was ≤3, which means the sample is questionable. Using two standard samples, the correlation between BactoScan TM and BactoCount TM was 0.9982, which means the results of total bacterial count measurement of both equipment were equivalent. Therefore, the standard samples manufactured in this study for quality control of total bacterial count using BactoScan TM and BactoCount TM in the raw milk could be applied to proficiency tests.
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KEYWORD
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Proficiency test, Quality control, Raw milk, Standard sample, Total bacterial count
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