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KMID : 1023520200430040227
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Korean Journal of Veterinary Service
2020 Volume.43 No. 4 p.227 ~ p.236
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Production of a transgenic pig expressing 3D8 single chain variable fragment (scFv) and its evaluation of PRRS resistant
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Lee Hwi-Cheul
Lee Gun-Sup
Kim Ji-Yoon
Yang Hyeon
Lee Bo-Ram
Park Mi-Ryung
Hwang In-Sul
Lee Poong-Yeon
Byun Sung-June
Kim Won-Il
Oh Keon-Bong
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Abstract
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In this study, we have developed 3D8 scFv transgenic pig (TG) by microinjection of fertilized one-cell pig zygotes (2.17%). The effect of 3D8 scFv TG on porcine reproductive and respiratory syndrome virus (PRRSV) resistance were evaluated through PRRSV VR2332 (1×10 3 TCID50/mL) challenge and transmission experiments. As a result, the average daily weight gain (ADWG) of TG increased compared to the wild type pigs (WT) in PRRSV challenge groups and the serum viremia levels of the TG was significantly lower than of WT on the 7 day and 21 day after infection, meaning that the viral shedding was suppressed by 3D8 scFv expression. These results suggest that the expression of 3D8 scFv in pig could suppress spreading of infected virus to pigs sharing a room.
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KEYWORD
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Transgenic pig, 3D8 scFv, PRRS, Evaluation
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