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KMID : 1023620040280030191
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Reproductive and Developmental Biology
2004 Volume.28 No. 3 p.191 ~ p.196
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Expression Comparison of the GFP Gene under the Controls of Several Internal Promoters in the Retrovirus Vectors with or without WPRE Sequence
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Kwon Mo-Seon
Kim Teo-An
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Abstract
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In this study, to construct more effective retrovirus system, we compared four internal promoters (RSV: Rous sarcoma virus, UbC: Ubiquitin C, β-actin, CMV: human cytomegalovirus) in the retrovirus vector by measuring GFP (green fluorescent protein) expression. The effect of WPRE (woodchuck hepatitis virus posttrans-criptional regulatory element) sequence on transgene expression was also investigated. Experiments were conducted with cells derived from three different species (human, pig and chicken) and evaluated the activity of each promoter and the effect of WPRE sequence by fluorometry and Western blotting. In all cells tested, RSV and CMV promoters were superior to UbC and β-actin promoters, and RSV promoter was the best one in chicken cells. The boosting effect of WPRE sequence on GFP expression was evident in human and porcine cells but not in chicken cells.
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KEYWORD
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Retrovirus vector, Promoter, GFP, WPRE
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