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KMID : 1023620040280030197
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Reproductive and Developmental Biology
2004 Volume.28 No. 3 p.197 ~ p.202
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Retrovirus Vector-Mediated Construction of Porcine Cell Lines Expressing Human PTH
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Jeong Jee-Yeon
Kim Nam-Hyung
Park Sung-Hwan
Kim Teo-An
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Abstract
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Human PTH (parathyroid hormone) is known to be efficacious for curing osteoporesis. In this study, we attempted to construct genetically modified porcine cell lines that can ultimately be used for donor cells in nuclear transfer-mediated transgenesis. By using retrovirus vectors carrying tetracycline-regulatory expression system and WPRE (woodchuck hepatitis virus posttranscriptional regulatory element) sequence, we could control PTH expression with tetracycline and boost the promoter activity. Considering that low or constitutive expression of the transgene has been one of major problems that needs to be solved in transgenic animal studies, our results could be helpful in successful production of transgenic pigs as bioreactors.
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KEYWORD
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hPTH, Tet-On system, retrovirus vector, WPRE
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