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KMID : 1023620040280030203
Reproductive and Developmental Biology
2004 Volume.28 No. 3 p.203 ~ p.207
Stable Transmission and Continuous Expression of Human Interleukin-10 Transgene in the Offspring of Transgenic Mice
Chung Jin-Woo

Koo Deog-Bon

Abstract
The transgenic mice carrying human Interleukin-10 (hIL-10) gene in conjunction with bovine (3 -casein promoter express hIL-10 in milk during lactation. In this study, stability of germ line transmission and expression of hIL-10 transgene integrated into host chromosome were monitored up to generation F8 of transgenic mice. When male mouse of generation F8 was crossbred with normal females, approximately half of offspring (50.9¡¾5.8%) were identified as transgenic mice. Generation F9 to F15 mice also showed similar transmission rates (66.0¡¾20.1%, 61.5¡¾16.7%, 41.1¡¾8.4%, 40.7¡¾20.3%, 61.3¡¾10.8%, 49.2¡¾18.8% and 43.8¡¾25.9%, respectively), implying that hIL-10 transgene can be transmitted stably up to long term generation in the transgenic mice. Expression levels of human IL-10 from milk of generation F9 to F14 mice were 3.6¡¾ 1.2 mg/ml, 4.2¡¾0.9 mg/ml, 5.7¡¾1.5 mg/ml, 6.3¡¾3.5 mg/ml, 6.8¡¾4.5 mg/ml and 6.8¡¾3.1 mg/ml, respectively, which was showed high-level expression compared with that of generation F1 (1.6 mg/ml) mice. In conclusion, our results suggest that transgenic mice can be continuously passed their transgenes to the progeny through the breeding program with the same productivity of human IL-10 protein in their milk.
KEYWORD
Transgenic mice, IL-10, Transmission, Expression
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