KMID : 1023620040280040261
|
|
Reproductive and Developmental Biology 2004 Volume.28 No. 4 p.261 ~ p.265
|
|
Assessment of Sperm Characteristics in Fresh and Frozen Semen of Miniature-Pig
|
|
Lee S. H.
Kim T. S. Cheong H. T. Yang B. K. Kim C. I. Park C. K.
|
|
Abstract
|
|
|
The present study was conducted to assess sperm characteristics in miniature-pig. The semen samples were transported to the laboratory at 17¡É within 3 hours after collection. The extended semen was stored at 17¡É, and sperm quality was evaluated at 0, 1, 3, 5 and 7 days after storage. The semen volume of miniature-pig (62¡¾22§¢) was significantly (p<0.05) lower than that of Duroc (155¡¾25§¢) and Yorkshire (154¡¾23§¢). Significant differences were also observed in sperm concentrations. During 3 days of storage, sperm viability did not differ among miniature-pig, Duroc and Yorkshire. However, the viability was significantly (p<0.05) lower in miniature-pig than in Duroc and Yorkshire semen after Day 3 of storage. In abnormality, acrosome intactness and intensity, there were no differences among miniature-pig, Duroc and Yorkshire semen. On the other hand, the viability of frozen-thawed sperm in miniature-pig was significantly (p<0.05) lower than in that of Duroc and Yorkshire. This study also examined CTC patterns in frozen-thawed spermatozoa. The rates of AR pattern were higher in miniature-pig than in Duroc and Yorkshire. However, no difference was found in F, B and AR patterns. The results of present study suggest that further research is necessary to develop of semen extender and freezing methods to improve sperm quality in miniature-pig.
|
|
KEYWORD
|
|
Miniature-pig, Semen characteristics, Frozen-thawing, CTC pattern
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|