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KMID : 1023620050290030145
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Reproductive and Developmental Biology
2005 Volume.29 No. 3 p.145 ~ p.147
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Optimization of Embryo Density and the Volume of Culture Medium for an Improvement of Mouse Parthenogenetic Embryo Development
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Roh Sangho
Choi Young-Joo
Min Byung-Moo
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Abstract
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Autocrine or paracrine mediators released by the early embryo are implicated in the support of embryonic development. Their mechanisms and optimal embryo density in the medium, however, are uncertain. This study was conducted to establish the optimal embryo density and culture medium volume in mouse parthenogenetic embryo culture. In experiment 1, culture of parthenogenetirally activated oocytes at a concentration of embryos/ significantly improved development to the blastoryst stage () compared with culture at the lower (e mbryos/) and the higher ( embryos/) concentration for 120 h when the oocytes were cultured in a 5 drop under mineral oil In experiment 2, the embryos cultured at a concentration of embryos/ in a 10 drop () showed significantly higher blastocyst rates than those in a 5 drop (). This study optimizes in vitro culture condition by modifying embryo density and the volume of culture medium It may give appropriate level of autocrine and/or paracrine factors to enhance viability and subsequent normal development of mouse parthenogenetic embryos in vitro.
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KEYWORD
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Parthenogenesis, Embryo density, Culture medium volume
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