|
KMID : 1023620050290030155
|
|
Reproductive and Developmental Biology
2005 Volume.29 No. 3 p.155 ~ p.162
|
|
|
Effects of Taurine and -Tocopherol Treatment during freezing on Sperm Characteristics and Function in Frozen-Thawed Porcine Semen
|
|
Chung Yung-Chai
Kim Chang-Keun
Pang Myung-Geol
|
|
|
Abstract
|
|
|
|
The present study evaluated whether an exogenous antioxidants, taurine and -tocopherol, could, when added to the freezing extender, improve the post-thaw sperm characteristics, function, the level of reactive oxygen species (ROS) generation, and the level of lipid peroxidation (LPO) in frozen-thawed porcine semen. CASA (computer-aided sperm analysis), HOST (hypoos-motic swelling test), chemiluminescence using luminol and lucigenin and the detection of malondialdehyde for LPO was performed in frozen-thawed porcine sper-matozoa. The results obtained in these studies are as follows. While no beneficial effects of taurine and -tocopherol supplementation were visible in motility, viability, acrosome reaction, tail swelling patterns, and the generation of of frozen-thawed porcine sper-matozoa, was decreased by all treatments except taurine 50mM treatment. In conclusion the taurine and -tocopherol treatments during freezing reduced generation of reactive oxygen species and production of malondialdehyde in frozen-thawed porcine semen, and the ROS savangers may minimize various damages of spermatozoa during freezing.
|
|
|
KEYWORD
|
|
|
Sperm, Freezing, ROS, Taurine
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|