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KMID : 1023620050290030163
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Reproductive and Developmental Biology
2005 Volume.29 No. 3 p.163 ~ p.168
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Lee J.H.
Kim C.K.
Chang Y.M.
Ryu J.W.
Park Min-Young
Chung Yung-Chai
Pang Myung-Geol
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Abstract
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The aim of this study was to investigate the changes in insulin-like growth factor-I (IGF-I) and growth hormone (GH) in serum, the quantitation of spermato-genesis and the comparable relationships among these measurements during pubertal period in New Zealand White male rabbits. To investigate the age-related testicular changes in DNA contents of spermatogenic cells, the fine-needle testicular biopsies from males aged 10 to 28 wks were evaluated by flow cytometry(FCM). Body weight increased significantly between the ages of 12 and 20 wks (P<0.05) and reached 3.4 kg at 28 wks of age. The highest serum IGF-I level (451.3ng/mL) was observed at 20wks of age (P<0.05) and thereafter remained stable at low levels. Serum GH level at 18 wks of age was 183.3 pg/mL which was significantly higher compared to the other ages (P<0.05), and the rising time in serum GH tend to be somewhat earlier than that of IGF-I. The relative percentage of It-cells in testicular cell compartments was at the age of 18 wks which significantly increased than those of 16-wk-old (P<0.05) and thereafter increased with the advance of age to . The percentage of 2C-cells in testis was at 18 wks of age which was significantly lower than at 16 wks old (P<0.05). The percentage of 4C-cells was constantly maintained except the at 18 wks of age. In conclusion, the results suggest that the puberty onset occurred at about the 18 wks of age and that the IGF-I and GH in serum during the pubertal period showed the age/growth-specific changes and these changes might be related to the spermatogenesis. The DNA FCM combined with fine-needle testicular biopsy could offer a very sensitive method to monitor the quantitative spermatogenic events related to the puberty onset.
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KEYWORD
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토끼, 춘기 발동, 정자 형성, Flow cytometry, IGF-I, Puberty, Rabbit, Spermatogenesis
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