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KMID : 1023620050290030169
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Reproductive and Developmental Biology
2005 Volume.29 No. 3 p.169 ~ p.174
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Effects of Culture Medium and Osmolarity on In Vitro Maturation of Follicular Oocytes and Development of Parthenogenetic Embryos in Porcine
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Kim M.K.
Park C. K.
Yang Boo-Keun
Cheong Hee-Tae
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Abstract
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This study was comducted to examine the effects of culture medium, and the osmolarity and osmotic change of the culture medium on in vitro maturation of porcine oocytes and developement of porcine parthenogenetic embryos. In Experiment 1, cumulus-oocyte complexes were matured in NCSU-23, mWM and mKRB, respectively, There was no difference in maturation rate() among groups. In Experiment 2, matured oocytes in each medium were activated and cultured for 6 days in the same media. Blastocyst formation rate was higher in NCSU-23() than those of others(, P<0.05). In Experiment 3, parthenogenetic embryos were cultured for 6 days in NCSU-23 with different osmolarity(300, 280 and 256 mOsmols) adjusted by NaCl. There were no differences in development rates to the blastocyst stage() among groups. In Experiment 4, activated oocytes were cultured for 2 days in NCSU-23 with 300, 280 and 256 mOsmols and then transferred to increased or decreased osmotic condition. Blastocyst formation rate was higher in a group which was transferred from the higher osmotic condition to the lowe. osmotic condition() than a contrary group( , (P<0.05). This result shows that the culture medium and the osmolarity of the culture medium affect the development of porcine parthenogenetic embryos, and the change of osmolarity from the higher condition to the lower condition at a certain developmental stage can enhance the development of porcine parthenogenetic embryos.
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KEYWORD
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Parthenigenetic embryos, In vitro culture, Osmolality, Porcine
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