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KMID : 1023620050290040207
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Reproductive and Developmental Biology
2005 Volume.29 No. 4 p.207 ~ p.212
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Effects of Genotypes on In Vitro Maturation and Fertilization of Frozen-Thawed Porcine Oocytes
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Jia Y. H.
Jin H. J.
Wee M. S.
Cheong H. T.
Yang B. K.
Park C. K.
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Abstract
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In the present study, we investigated the effects of genotypes on in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes. The porcine cumulus-oocyte complexes (COCs) were divided into four groups according to whether they were: (1) in vitro matured; (2) cryopreserved and in vitro matured; (3) in vitro fertilized and (4) cryopreserved, and in vitro fertilized. Maturation of porcine COCs was accomplished by incubation in NCSU23 medium. Immature oocytes were cryopreserved by Open Pulled Straws (OPS) method according to Vajta et al., (1998). Oocytes stained by Acetic-Orcein method were observed under the microscope. DNA extracted from the ovaries was analyzed by RAPD (random amplified polymorphic DNA) and SSCP (single strand conformational polymorphisrrt) method. The rates of oocytes maturation and fertilization were significantly high in AA genotype. The results indicated that in vitro maturation and fertilization in porcine fresh/frozen-thawed oocytes may be affected by genotypes in pigs.
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KEYWORD
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Porcine oocytes, Cryopreservation, Genotype, IVM-IVF
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