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KMID : 1023620050290040241
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Reproductive and Developmental Biology
2005 Volume.29 No. 4 p.241 ~ p.245
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In Vitro Maturation of Round Spermatids Using Porcine Oviduct Epithelial Cell Monolayer Condition Medium
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Jabed Md. Anower
Kamal Tania
Lee Seung-Min
Kim Byung Ki
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Abstract
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Porcine oviduct epithelial cells (POEC) are widely used in co-culture experiments to improve early embryonic development, in vitro fertilization in embryo transfer programs for domestic animals and in vitro maturation of immature germ cells. POEC were mechanically isolated and cultured in tissue culture medium 199. Cells grew continuously, and confluent monolayers were formed after 7 days. After forming confluent monolayer of epithelial cells, supernatant was collected as the condition medium for maturing round spermatids in vitro. Round spermatids were also separated mechanically and cultured in the POEC condition medium. In this study we observed that of round spermatid cultured were matured into elongating spermatid after 24 h, and about of round spermatid cultured showed complete elongation (elongated spermatid) within h of in vitro culture. No further development was observed within h and transformed cells lost their viability after 72 h. These preliminary findings suggest that the condition medium from POEC may be possible to overcome the round spermatid block by improving the milieu of culture system.
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KEYWORD
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Oviduct, Epithelial cell, Monolayer, Round Spermatid, In vitro maturation
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