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KMID : 1023620050290040253
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Reproductive and Developmental Biology
2005 Volume.29 No. 4 p.253 ~ p.257
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Analysis of Membrane Integrity and Mitochondrial Activity in Fresh and Cryopreserved Boar Sperm Using Flow Cytometry
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Park C. S.
Li Z. H.
Sung N. D.
Jin D. I.
Cong P. Q.
Kim E. S.
Song E. S.
Yi Y. J.
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Abstract
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This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than of fresh sperm washed with mTLP-PVA medium at exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane and nonfunctional mitochondrion in the mTLP-PVA and BTS washing media at . There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at washing temperature than at washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.
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KEYWORD
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Membrane integrity, Mitochondrial activity, Boar sperm, Flow cytomety
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