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KMID : 1023620060300010001
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Reproductive and Developmental Biology
2006 Volume.30 No. 1 p.1 ~ p.5
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Development of Porcine Parthenogenetic Oocytes Activated with Different Combination of Chemicals
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Seo Jin-Sung
Hwang In-Sun
Kim Se-Woong
Park Hyo-Suk
Kim Dong-Hoon
Yang Byoung-Chul
Yang B.-S.
Im Gi-Sun
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Abstract
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Artificial activation of oocytes is a prerequisite for the successful cloning by nuclear transfer. This study investigated the effect of the different combination of activation agents such as electric pulse (E), thimerosal (Thi) + dithiothreitol (DTT), 6-dimethylaminopurine (6-DMAP) or cycloheximide (CH) on the developmental ability of porcine embryos derived from parthenogenetic activation (PA). PA embryos activated with chemicals showed significantly higher developmental rate to the blastocyst stage compared to the embryos activated with E alone ( vs. 18.0%, respectively). Of chemicals, Thi + DTT supported higher development to the blastoryst stage (28.1%). There was no significant difference in 1 pronucleus (PN) formation rate , but 2PN formation rate was significantly higher in PA embryos with additional activation using chemicals . In conclusion, this study shows that chemical activation after electric pulse can increase the development of porcine PA embryos.
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KEYWORD
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Porcine, Parthenogenetic activation, Thi+DTT, 6-DMAP, Cycloheximide
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