|
KMID : 1023620060300010007
|
|
Reproductive and Developmental Biology
2006 Volume.30 No. 1 p.7 ~ p.11
|
|
|
Effects of Hormone and Na-Pyruvate on the In Vitro Maturation of Canine Oocytes
|
|
Kim Cheon-Ho
|
|
|
Abstract
|
|
|
|
This study was conducted to examine the effects of hormone and sodium pyruvate on in vitro maturation of canine oocytes. Canine oocytes were collected from the ovaries of dogs and cultured in NCSU-37 medium with hormones and sodium pyruvate for 72 hr. Oocytes matured to the metaphase II (MII) stage were observed only from estradiol , and the presence of gonadotropin did not improve the nuclear maturation. No oocytes were developed to the MII stage when was added to medium during the first 6 and 24 hrs of culture period. The presence of during the whole culture period enhanced the nuclear maturation to the MII stage (6.0%, P<0.05). High concentration of sodium pyruvate (2.5 mM) slightly enhanced the nuclear maturation to the metapahse I (HMI) stage, but not the MII stage. the result of the present study shows that the presence of during the whole culture period of 72 hr enhances the maturation of canine oocytes to the M stage, but sodium pyruvate does not affect the nuclear maturation of the canine oocytes.
|
|
|
KEYWORD
|
|
|
IVM, Hormone, Na-pyruvate, Canine oocyte
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|