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KMID : 1023620060300010021
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Reproductive and Developmental Biology
2006 Volume.30 No. 1 p.21 ~ p.26
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Effect of Various Supplements on Embryo Development and Quality of Bovine Embryos during In Vitro Maturation
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Park Hum-Dae
Jang Mi-Jin
Park Yong-Soo
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Abstract
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This study was examined the effects of concentrations of polyvinylpyrrolidone(PVP) and supplementation of EGF, cysteine and PVP during in vitro maturation on the development of bovine embryos. In experiment 1, 0.1 to 3.0% PVP was supplemented to IVM medium before IVF. The development rates to the blastocyst stage was significantly higher in 0.5% PVP group than 3.0% PVP group (P<0.05). In experiment 2, EGF, rysteine and PVP were supplemented to IVM medium. The hight cleavage rate was obtained from cysteine group, but blastocyst formation rates did not differ among groups. The highest total cell number and inner cell mass (ICM) cell number were observed in cysteine group. In PVP group, ICM cell number was significantly low than those of cysteine and control groups (P<0.05). After embryo transfer, pregnancy rate was significantly low in PVP group compared to other groups (P<0.05). These results indicate that the supplementation of PVP in IVM medium support the embryo development, but has a deteriorate effect on the blastocyst quality.
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KEYWORD
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Bovine, IVM, PVP, EGF, Cysteine
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