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KMID : 1023620060300010035
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Reproductive and Developmental Biology
2006 Volume.30 No. 1 p.35 ~ p.40
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Effects of Embryo Developmental Stage and Superoxide Dismutase on the Survival of Frozen-Thawed Porcine Embryos by Open Pulled Straw (OPS) Method
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Lee Sang-Young
Park Choon-Keun
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Abstract
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This study was performed to investigate the effects of embryo developmental stage and superoxide dismutase (SOD) on the survival of frozen-thawed porcine embryos by open pulled straw(OPS) method. Porcine IVF blastocysts were frozen-thawed by OPS method and cultured for 48 h under the existence of SOD. There are no significant differences in the proportions of normal morphology among the early, mid- and expanded blastoryst stages . After culture of embryos, the developmental rates to the expanded blastocyst stage(38.7%) were significantly higher than those of other stages (P<0.05). The proportions of expanded and hatched embryos were higher in medium with 1 unit/ml SOD than 0 and 10 units/ml of SOD. The result indicates that OPS method can use for the pig embryo cryopreservation, especially for the late stage blastocysts. SOD may can reduce the demage of frozen-thawed porcine embryos.
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KEYWORD
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OPS, SOD, Pig blastocyst, Survival ability
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