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KMID : 1023620060300010041
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Reproductive and Developmental Biology
2006 Volume.30 No. 1 p.41 ~ p.45
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Activation by Combined Treatment with Cycloheximide and Electrical Stimulation of In-Vitro Matured Porcine Oocytes Improves Subsequent Parthenogenetic Development
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Naruse Kenji
Kim Beak-Chul
Park Tae-Young
Choi Su-Min
Park Chang-Sik
Jin Dong-Il
Kim Hong-Rye
Shin Young-Min
Chang Suk-Min
Lee Hye-Ran
Tarte Vaishali
Quan Yan-Shi
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Abstract
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Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to calcium ionophore for 2 min with cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development.
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KEYWORD
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Porcine oocytes, Cycloheximide, Electrical stimulation, Parthenogenetic development, In vitro culture
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