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KMID : 1023620060300010059
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Reproductive and Developmental Biology
2006 Volume.30 No. 1 p.59 ~ p.64
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Establishment of the Convenient Boar Semen Freezing Method and Assessment of Viability in Frozen/Thawed Boar Semen
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Kim Seong-Kon
Jang Hyun-Yong
Park Dong-Heon
Park Choon-Keun
Cheong Hee-Tae
Kim Choung-Ik
Yang Boo-Keun
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Abstract
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This study was conducted to establish a convenient freezing method of boar semen. Boar semen was cooled until for 3 hrs using cell freezer and loaded into straws. Semen straws were frozen in different steps in strofoam box filled with . Highest sperm viability (54.0%) was obtained by 1-step freezing(holding at 10 cm height from the surface of for 10 min). Sperm viability increased by holding at for 10min (74.0%, P<0.05). In thawing regime, sperm viability was significantly higher in group than in group. The sperm characteristics did not differ between 1-step and 3-step. After IVF using frozen-thawed boar semen, developmental rate of embryos to the morula+blastocyst stage was in 1-step freezing group than that of 3-step freezing group (27.5 vs 14.7%, P<0.05). The result shows that the 1-step freezing with holding at for 10min before plunging into is a convenient and easy freezing method for boar semen.
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KEYWORD
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Frozen/thawed boar semen, Acrosome intactness, Semen quality, Embryo development
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